Siderophore detection: It provides a quick and sensitive test for the detection of iron binding peptides, i.e. Siderophores or synthetic analogues, for therapeutic application
Iron Chelation: Assessment of chelation ability of novel synthetic chelation molecules for therapeutic application in iron overload.
Antimicrobial Discovery: It provides an easy method to screen for compounds which can act as potential inhibitors of iron uptake via inhibition of siderophore generation.
The SideroTec assay is a colorimetric test for use in the detection of siderophores secreted by bacteria or fungi, or for the assessment of synthetic iron chelators. The test can be used with liquid culture either directly or following filtration to remove microorganism. The test may also be used with other aqueous based liquids.
Feedback from β site trials
I do think the kit would be of interest to many in the siderophore community, especially if it is quantitative and has a decent dynamic range. Multinational Pharmaceutical Company
Overall, we are very pleased with its sensitivity, rapid analysis time and its ease of use. We have tried many times to make the siderophore assay solution from literature recipes without much success-certainly not the sensitivity of your assay. American Type Culture Collection
We have run a few of our chelators using your kit and found it useful for ranking the compounds in order of increasing iron binding affinity at pH 7.4. Overall the assay is much faster and more convenient than what we usually do. Apopharma
Your assay gave the precise concentration of the siderophores produced by our reference strain(mutant of Mycobacterium smegmatis). This was confirmed by HPLC. We had positive feed-back on the siderophore work anddetection kit during ESBES. IBB-Institute for Biotechnology and Bioengineering, Portugal
Purified Fungal Siderophores: EmergenBio supplied purified fungal siderophores in both ferrated and non-ferrated format. These are generated under iron-free conditions.